ASMS 2017

Check out what you missed at the annual conference hosted by American Society for Mass Spectrometry

Discover how the advancing technologies of Prosolia are making mass spec better!

Prosolia is changing what’s possible in the lab and on the field. From uncovering disease pathology, to novel drug screening methods, and advancing how researchers trace disease pathology, your won’t look at mass spec the same way again.

Did you miss us at ASMS? As predicted, the breakfast seminar was a major highlight of ASMS—the talk was standing room only!

Dr. Liz Dhummakupt and Nick Manicke both gave incredible presentations. Learn more and download their presentations below.

Direct Analysis of Chemical Warfare Simulant Aerosol by Paper Spray Mass Spectrometry

Elizabeth S Dhummakupt

Dr. Liz Dhummakupt discussed the implications of utilizing PaperSpray ionization mass spectrometry as a rapid alternative platform to analyze aerosolized chemical warfare agents (CWAs) requiring no sample prep with benefits including improved resolution and sensitivity.

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There is a growing need for improved analysis of aerosolized chemical warfare agents (CWAs). Current detection methods (ion mobility spectrometry, colorimetric paper, etc.) suffer from poor resolution, detector saturation and sensitivity to temperature and humidity. Paper spray ionization mass spectrometry (PS-MS) offers a rapid alternative platform that requires no sample preparation. Aerosolized CWA simulants, trimethyl phosphate (TMP), dimethyl methylphosphonate (DMMP), and diisopropyl methylphosphate (DIMP), were captured in the traditional manner (‘gold-standard’) by passing the air through a glass fiber filter disk or by capturing it directly onto Prosolia’s paper spray cartridge consumable. Both approaches were performed in tandem and rigorously compared.

CWA simulants at varying concentrations were prepared and aerosolized using an in-house built aerosol chamber. To facilitate the aerosol capture onto the paper spray cartridges a custom 3D printed holder was designed and built. The air flow through each of the collection devices (PS-MS vs. Gold-Standard) was maintained at 1.5 L per minute to assure the same volume of air sampled across methods. After collection, the PS cartridges were analyzed using the Prosolia Velox 360 on a Thermo Fisher Orbitrap Elite MS; the filtration disks were extracted and analyzed on an Agilent 6490 Triple Quad MS.

The results from each capture and detection strategy were compared. In both instances, each approach yielded linear calibration curves with R2-values between 0.98-0.99 for each compound and similar limits of detection (LOD) in terms of disbursed aerosol concentration. Therefore, the paper spray method generates comparable results to the gold-standard extraction method, but the PS analysis does not require the extraction step. Based off these results, the sampling efficiency for each technique was calculated. While the glass fiber filter disk has a higher sampling efficiency (~40%), the paper spray method produces analogous results even with a lower sampling efficiency (~1%).

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Dr. Elizabeth S Dhummakupt received her bachelor’s degree in chemistry and biology from Vanderbilt University in 2010. She attended graduate school at the University of Florida in the Department of Chemistry. While there, she worked under the direction of Dr. Richard Yost, and her Ph.D. research focused on analysis of illicit drugs in dried blood spots using MALDI and paper spray ionization mass spectrometry. After graduating in 2015, she was a post-doctoral researcher at UF for Dr. Timothy Garrett with projects focusing on development of uHPLC-HRMS methods for metabolomics. Dr. Dhummakupt is currently a National Research Council post-doctoral researcher at Edgewood Chemical Biological Center in Maryland where she is developing methods for analysis of chemical warfare agents via paper spray ionization MS.

Laboratory Statement:

The ECBC mass spectrometry facility is led by Dr. Trevor Glaros and is comprised of a multidisciplinary team of scientists whose core competencies range widely encompassing proteomics, metabolomics, molecular biology, and instrument development. Chemical and biological agents pose a serious challenge to public health as well as to military personnel worldwide. As such, our primary mission, using mass spectrometry as a tool, is to aid in the development of novel of therapeutics and detection strategies to protect our warfighters.

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Clinical and Forensic Applications of Paper Spray MS

Nick Manicke

Learn how PaperSpray® technology has enabled targeted quantitative analysis of drugs within a single cartridge from Nick Manicke as he discusses this and other emerging applications improved through mass spectrometry.

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Recent applications of paper spray MS will be described, including therapeutic drug monitoring and drug screening. The therapeutic drug monitoring application consists of targeted quantitative analysis on a triple quad mass spectrometer. The drug screening work involves screening for over 100 different drug targets from a single paper spray cartridge on a Q Exactive mass spectrometer. Results for paper spray screening of post-mortem blood samples will be included. Emerging applications performed using novel paper spray cartridges will also be presented. This will include analyzing designer drugs such as fentanyl analogs and spice cannabinoids on a paper spray cartridge with built-in solid phase extraction. Another new area to be presented will be targeted detection of plasma proteins from an all-in-one cartridge.

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Manicke joined the faculty of the Department of Chemistry and Chemical Biology at Indiana University-Purdue University Indianapolis (IUPUI) in August of 2013 after spending the previous two years as the principal scientist for a Purdue University spin-out company founded on technology Manicke co-invented as a post-doctoral researcher at Purdue. Manicke earned his Ph.D. in analytical chemistry at Purdue University in 2009 under the supervision of R. Graham Cooks. He earned his undergraduate degree in biochemistry from the University of Evansville (Indiana). Manicke has authored or co-authored over 30 peer-reviewed scientific papers on mass spectrometry including recent papers in Analytical Chemistry, Clinical Chemistry, and JASMS. He has also delivered podium presentations at the ASMS Conference on Mass Spectrometry and at the Mass Spectrometry: Applications to the Clinical Laboratory (MSACL) conference.

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Oral Presentations

MOD 08:30AM Sagamore 4: Paper Spray Mass Spectrometry for the Detection of Chemical Warfare Agent Simulants
Elizabeth S. Dhummakupt [1, 2]; Josiah McKenna [3] ; Theresa Connell [4] ; Paul Demond [4]; Dennis B. Miller [1] ; Nicholas E. Manicke [3] ; J Michael Nilles [4] ; Trevor Glaros [1] ;

[1] US Army ECBC, Aberdeen Proving Ground, MD; [2] National Research Council, Washington, DC; [3] Indiana University-Purdue University Indianapolis, Indianapolis, IN; [4] Excet, Inc., Springfield, VA
WOD 09:30AM Sagamore 4: Endometrial Cancer Tissue Identification by Desorption Electrospray Ionisation (DESI) Imaging
Olivia Raglan [1], 2; Luisa Doria [1] ; Renata F. Soares [1] ; James McKenzie [1] ; Zoltan Takats [1] ; Maria Kyrgiou [1, 2];

[1] Imperial College London, London; [2] Imperial College Healthcare NHS Trust, London, UK
WOF 3:50PM Wabash: Optimized processing workflow for improved information recovery from large-scale MSI data
Kirill Veselkov [1]; Jonathan Sleeman [2]; Emmanuelle Claude [3]; Ivan Laponogov [1]; Mark Towers [3]; Robert Tonge [3]; Jeremy K Nicholson [1]; Zoltan Takats [1]; James Langridge [3]

[1] Imperial College London, London, United Kingdom; [2] Center for Biomedicine and Medical Technology, Mannheim, Germany; [3] Waters Corporation, Wilmslow, United Kingdom
ThOF 2:30PM Wabash: Tumor Margin Determination during Neurosurgery using DESI
R. Graham Cooks [1]; Valentina Pirro [2]; Clint Miles Alfaro [2]; Alan Jarmusch [2]; Eyas M Hattab [3]; Aaron A Cohen-Gadol [4]

[1] Purdue University, West Lafayette , IN; [2] Purdue University, West lafayette, Indiana; [3] Indiana University, Bloomington, IN; [4] Indiana University−Purdue University Indianapolis, Indianapolis, Indiana


MP006: Innovations in Liquid Microjunction Surface Sampling Probe Mass Spectrometry
Louis Searcy [1]; Arun Wanchoo [2]; Cuong Nguyen [2]; Timothy J. Garrett [2]; Richard A Yost [2]

[1] University of Florida, Gainesville, FL; [2] University of Florida, Gainesville, FL
MP286: A Multi-Center Study Using Desorption Electrospray Ionization Mass Spectrometry Imaging as A Robust Tool for Breast Cancer Diagnosis
Jialing Zhang [1]; Andréia M Porcari [2]; Raquel R Peres [2]; Kyana Garza [1]; Jonathan Young [1]; John Lin [1]; Rob Tibshirani [3]; Geisilene R Paiva [2]; Wendong Yu [4]; Chandandeep Nagi [4]; Stacey Carter [4]; Luís Z. Sarian [2]; Marcos N. Eberlin [2]; Livia S. Eberlin [1]

[1] The University of Texas, Austin, TX; [2] State University of Campinas, Campinas, Brazil; [3] Stanford University, STANFORD, California; [4] Baylor College of Medicine, Houston, TX
MP290: Molecular Characterization and Diagnosis of Endometriosis to Aid in Surgical Resection using Ambient Ionization Mass Spectrometry
Clara Feider [1] ; Suzanne Ledet [2] ; Michael T. Breen [2] ; Livia S. Eberlin [1]
[1] University of Texas at Austin, Austin, TX, United States ; [2] Seton Medical Center, Austin, TX, United States
MP291: Identification of Biomarkers for Mild Traumatic Brain Injury in Tissue and Biological Fluids using DESI and PaperSpray Ionization with HRAM
Joseph H. Kennedy [1]; Riyi Shi [2]; Marcela Cruz-Haces [2]; Jonathan Tang [2]; Brian Laughlin [1]; Justin Wiseman [1]

[1] Prosolia, Inc., Indianapolis , IN; [2] Purdue University, West Lafayette, IN
MP297: Exploration of Desorption Electrospray Ionization (DESI) and Touch Spray (TS) Mass Spectrometry for squamous cell carcinoma (SCC) diagnosis and prognosis
Cedric DHue [1]; Michael Moore [2]; Don-John Summerlin [3]; Alan Jarmusch [4]; R. Graham Cooks [5]

[1] West Lafayette, INDIANA; [2] University of California, Davis, Davis, California; [3] Indiana University-Purdue University Indianapolis, Indianapolis, IN; [4] University of California, San Diego, San Diego, California; [5] Purdue University, West lafayette, Indiana
MP298: Tissue microarrays analysis using DESI-MSI
Anna K Mróz; Luisa Doria; James McKenzie; Francesca Rosini; Hiromi Kudo; Zoltan Takats; Renata Soares

Imperial College London, London, United Kingdom
TP159: Application of paper spray differential ion mobility mass spectrometry for pesticide products screening
Khang To

University of North Carolina, Chapel Hill, NC
TP329: Multiscale mass spectrometry imaging of colorectal cancers by MALDI, DESI and SIMS
Rory T Steven [1]; Adam J Taylor [1]; Spencer A Thomas [1]; Alan M Race [1]; Gregory Hamm [2]; Nicole Strittmatter [2]; Rasmus Havelund [1]; Renata F Soares [3]; Andrew D Campbell [4]; Owen J Sansom [4]; Richard J. A. Goodwin [5]; Zoltan Takats [3]; Josephine Bunch [1]

[1] National Physical Laboratory, Teddington, United Kingdom; [2] AstraZeneca R&D, Cambridge, United Kingdom; [3] Imperial College London, London, London; [4] Beatson Institute for Cancer Research, Bearsden, United Kingdom; [5] Astrazeneca, Cambridge, United Kingdom
WP005: Coupling PaperSpray Technology to High-End Orbitrap Mass Spectrometry
Kerry M. Hassell [1]; Maria C. Prieto Conaway [2]

[1] ThermoFisher Scientific, Somerset, NJ; [2] ThermoFisher Scientific, San Jose, CA
WP008: Improving Quantification Using PaperSpray Ionization coupled to Ion Trap Mass Spectrometry for Clinical Research
Cornelia L. Boeser, Linfan Li, Jae Schwartz

ThermoFisher Scientific, San Jose, CA
WP010: Molecular Discrimination of Follicular Thyroid Adenomas and Carcinomas using Ambient Ionization Mass Spectrometry
Rachel J. DeHoog ; Jialing Zhang ; Elizabeth Alore ; Wendong Yu ; Rob Tibshirani ; Anton F Engelsman ; Stan B Sidhu ; James Suliburk ; Livia S. Eberlin

University of Texas at Austin, Austin, TX, United States ; Baylor College of Medicine, Houston, TX ; Stanford University, Stanford, California ; Royal North Shore Hospital, Sydney, NSW, Australia ; University of Sydney, Sydney, NSW, Australia
WP020: Automated PaperSpray sample preparation using a robotic autosampler for clinical research
Steven L. Reeber, Lucas Lüthy (CTC Analytical AG), Cornelia Boeser, John Glazier

Thermo Scientific, San Jose, CA
WP022: Distinguishing Non-Small Cell Lung Cancer Subtypes by Desorption Electrospray Ionization Mass Spectrometry
Alena Bensussan [1]; Tanweer Zaidi [2]; Ruth Katz [2]; Erik Cressman [2]; Livia S. Eberlin [1]

[1] University of Texas at Austin, Austin, TX; [2] MD Anderson Cancer Center, Houston, TX
WP025: Methodologies for Investigating Neurodegenerative Disorders Using a Liquid-Microjunction Surface Sampling Probe
Emily L. Gill; Megan Marks; Richard A. Yost; Vinata Vedam-Mai; Timothy J. Garrett

University of Florida, Gainesville, FL
WP027: PaperSpray High Resolution Accurate Mass Spectrometry for Quantitation of NSAID Drugs in Equine Plasma
David J. Borts [1] and Joseph H. Kennedy [2]

[1] Iowa St. University, Aimes, IA; [2] Prosolia, Inc., Indianapolis , IN
WP118: Lipid Characterization in Genetically Engineered Yarrowia lipolytica by Desorption Electrospray Ionization Mass Spectrometry
John Lin [1]; Lauren T. Cordova [2]; Hal S. Alper [2]; Livia S. Eberlin [1]

[1] Department of Chemistry, The University of Texas at Austin, Austin, TX; [2] McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX
WP276: Data directed mass spectrometry imaging – real time decision making during automated analysis of sample slide batches
Emrys Jones [1] [2]; Emmy Hoyes [1]; Lukasz Migas [3]; Fiona Henderson [4]; Praveen Harapanahalli [1]; Richard Chapman [1]; Adam McMahon [3]; Steven Pringle [1]; Zoltan Takats [2]

[1] Waters Corporation, Wilmslow, United Kingdom; [2] Imperial College London, London, London; [3] University of Manchester, Manchester, United Kingdom; [4] University of Manchester, Manchester, United Kingdom
ThP133: PaperSpray Technology for the Study of Selective High Affinity Ligands (SHALs) and their Metabolites
Maria C. Prieto Conaway [1], Kerry M. Hassell [2], Rod Balhorn [3]

[1] Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA 95134; [2] Thermo Fisher Scientific, 265 Davidson Ave., Somerset, NJ 08873; [3] SHAL Technologies Inc, 15986 Mines Road, Livermore, CA 94550